ffpe rabbit mab  (Cell Signaling Technology Inc)

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: IL13Ra2 staining extent in BSG tissues. ( A ) Typical images of multiple fluorescence staining and spectral resolution of BSG FFPE tumor tissues (20×, all scale bars: 50μm); ( B ) expression of IL13Ra2, H3.3-K27M, Ki67, CD133, CD4, and HLA-1; ( C ) expression of H3.3K27M in H3F3A-mutant and wild-type tissues.

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Staining, Fluorescence, Expressing, Mutagenesis

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: IL13Ra2 expression in different BSG subtypes of the TMA cohort. ( A ) IL13Ra2 expression of BSG tissues from patients of different genders; ( B ) IL13Ra2 expression of BSG tissues from patients of different ages; ( C ) expression of IL13Ra2 in different locations of BSG tumor tissues; ( D ) expression of IL13Ra2 in DIPG and non-DIPG tumor tissues; ( E ) expression of IL13Ra2 in H3F3A-wt and H3F3A-mt tumor tissues; ( F ) expression of IL13Ra2 in BSG tissues of different WHO grades (ns p > 0.05, * p < 0.05, ** p < 0.01, **** p < 0.0001).

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Expressing

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: Co-staining of IL13Ra2 with H3.3-K27M, Ki67, and CD133. Images showing co-expression of IL13Ra2 and H3K27M ( A ), Ki67 ( B ), and CD133 ( C ) in BSG tumor tissues. The linear regression results between IL13Ra2 expression and H3K27M ( D ), Ki67 ( E ), and CD133 ( F ) expression in BSG tumor tissue. Comparison of the positive rates of H3K27M ( G ), Ki67 ( H ), and CD133 ( I ) in IL13Ra2-positive and negative cells from different tumor samples. Comparison of the positive rates of IL13Ra2 in H3K27M-positive/H3K27M-negative ( J ), Ki67-positive/Ki67-negative ( K ), and CD133-positive/CD133-negative ( L ) tumor cells across different tumor tissues (**** p < 0.0001).

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Staining, Expressing, Comparison

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: Kaplan–Meier survival analysis of patients with BSGs in the TMA cohort. Kaplan–Meier survival analysis results of BSG patients with different IL13Ra2 expressions ( A ), different H3K27M mutation statuses ( B ), DIPG and non-DIPG types ( C ), and different WHO grades ( D ).

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Mutagenesis

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: Heatmap of the BA cohort. A heatmap shows the differences in functional pathways and cellular components between the IL13Ra2-high group and IL13Ra2-low group of tumor tissues of the BA cohort. *1: WP_REGULATION_OF_SISTER_CHROMATID_SEPARATION_AT_THE_METAPHASEANAPHASE_TRANSITION; *2: INHIBITION_OF_THE_PROTEOLYTIC_ACTIVITY_OF_APC_C_REQUIRED_FOR_THE_ONSET_OF_ANAPHASE_BY_MITOTIC_SPINDLE_CHECKPOINT_COMPONENTS.

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Functional Assay, Inhibition, Activity Assay

Journal: Cancers

Article Title: Expression of Interleukin-13 Receptor Alpha 2 in Brainstem Gliomas

doi: 10.3390/cancers16010228

Figure Lengend Snippet: IL13Ra2 expression in BSGs of the BA cohort. Comparison of IL13Ra2 transcription level in tumor tissues with different H3F3A statuses ( A ), WHO grades ( B ), and methylation subtypes ( C ) in the BA cohort. The linear regression analysis results for IL13Ra2 transcription level and H3.3 ( D ), Ki67 ( E ), and CD133 ( F ) transcription levels in the BA cohort. ( G ) Kaplan–Meier survival analysis results of the IL13Ra2-high group and IL13Ra2-low group in the BA cohort (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Multiplex immunofluorescence staining (PerkinElmer, Waltham, MA, USA) was performed on FFPE slides for IL13Ra2 (1:400; #85677; CST, Danvers, MA, USA), H3.3 (1:800; #74829; CST, Danvers, MA, USA), Ki67 (1:400; ab70328; Abcam, Waltham, MA, USA), CD133 (1:400; #64326; CST, Danvers, MA, USA), CD4 (1:400; ab133616; Abcam, Waltham, MA, USA), HLA-1 (1:400; ab16667; Abcam, Waltham, MA, USA) and CD8 (1:200; ab4055; Abcam, Waltham, MA, USA).

Techniques: Expressing, Comparison, Methylation